Journal of Reproductive Endocrinology & Infertility

Histological Evidence and Proliferative Potential of the Contralateral Testis and Epididymis in Rats with Unilateral Testicular Torsion

We investigated histological changes in the contralateral testis and epididymis and proliferative potential of constituent cells of the contralateral testis and epididymis by silver staining of argyrophilic nuclear organizer regions (Ag-NORs) after unilateral testicular torsion (UTT). Fifty adult male rats were divided into 5 groups each containing 10 rats. Torsion operations were performed by rotating the left testes 720° in a clockwise direction. Torsion duration was 1, 4, 12 and 24 hrs that was lied in group 1 to group 4, respectively. Then, de-torsion operations were performed and 24 hrs. Later, histopathological changes in the contralateral testes and epididymis were investigated and compared to group 5(control). Control group underwent a sham operation of the left testis. In histologic examination, Johnson’s scores of right testes in group 3 and group 4 were different from other groups (P<0.001). In all groups, histological changes in the contralateral testes and epididymis were observed. Also, there was no significant difference in subjective Ag-NOR pattern assessment for constituent cells in the right testes and epididymis between different groups (P>0.05). Our findings demonstrate that UTT results in contralateral testicular and epididymis damage but no the quantitative changes in the rapidity of cell proliferation in cytohistological preparations.

Author(s):

Shahryar Amirhasani , Seyed Mohammad Hosseinipanah , Seyed Farshad Heidari , Seyed Habibollah Mosavibahar , Arash Dehghan , Hossein Mahjoub and Mohammad Taghi Goodarzi

Abstract | Full-Text | PDF

Share this